Online Event
September 01-03, 2022 | Online Event
GPMB 2022

Ribosomal Protein Genes Mediate Stress Tolerance in Crops

Anusree Saha, Speaker at Plant Science Conferences
University of Hyderabad, India
Title : Ribosomal Protein Genes Mediate Stress Tolerance in Crops

Abstract:

Ribosomal proteins are integrally associated with ribosomes, and they play important roles in mRNA and tRNA recognition, ribosome subunit assembly, transport, and stabilization. Apart from the canonical roles, several evidences suggest extraribosomal attributes of specific ribosomal proteins in various systems. Not much was known about the stress-responsive roles of RP genes until our research group identified activation of two ribosomal protein large subunit genes (RPL6 and RPL23A) while screening activation tagged mutant populations of rice for high water use efficiency. This led us to analyse the differential expression pattern of all RP (RPL and RPS) genes under simulated abiotic and biotic stress conditions, along with several in-silico analyses on their promoter sequences, gene structure, protein properties and phylogeny. Based on the expression analysis, we could shortlist some of the potential candidate RPL and RPS genes with high expression patterns under most stressed conditions. Further, we functionally characterized one of the large subunit genes (RPL6), and utilising molecular and physiological data, we validated the salt-tolerant role of RPL6 in independent transgenic rice lines overexpressing the gene. We were keen on finding the differential expression patterns of all the RPL and RPS genes in susceptible and resistant rice plants infected with Brown plant hopper and Gall midge at different time points post-infection. Hence, we reported a transcript analysis of the complete RP gene family in susceptible (BPT-5204) and resistant (RPNF05) indica rice genotypes challenged by two major insect pests, BPH and GM. We have also identified the candidate RP genes specifically or commonly upregulated in one or both the genotypes. Based on our extensive analysis of expression pattern RP genes under various conditions mimicking abiotic and biotic stress conditions, we could come up with a hypothesis that some of the RPL and RPS genes that were remarkably upregulated can be considered as significant RP genes essential for maintaining the integrity of the ribosomal machinery, ensuring sustained and undisturbed protein synthesis even under stress. As opposed to the general perception of the Ribosomal protein genes with their involvement in the growth and developmental functions, this study provides a strong basis for the possible involvement of RP genes in conferring abiotic and biotic stress tolerance in rice. The extent of research done in this area could only reveal the tip of the iceberg, and more thorough studies need to be done to understand the extra-ribosomal functions of plant RP genes completely.

Biography:

Anusree Saha completed her B.Sc (Botany) and M.Sc (Plant Biology and Biotechnology) from Presidency College, Kolkata and University of Hyderabad respectively. She joined University of Hyderabad for her doctoral studies in 2015 and successfully defended her doctoral thesis in Feb, 2022. During her PhD, she has published 2 first authored and 6 co-authored research articles in international journals. She got selected for Newton Bhabha PhD Placement and worked with Prof. Christine Raines at University of Essex, UK for four months in 2017. Currently, she is working as an Assistant Professor (Dept. Of Botany) at Raja Narendra Lal Khan Women’s college (Autonomous), West Bengal, India.

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