In vitro micropropagation of a salinity tolerant clonal rootstock issued from interspecific cross between glycophyte Solanum lycopersicum and halophyte Solanum chilense

The purpose of this study is to obtain quality salinity tolerant interspecific clonal rootstocks (Jupafor-1) through in vitro micropropagation to be grafted on tomatoes. This study is aimed at finding the optimal combinations and concentrations of growth regulators for the micropropagation of a salinity-tolerant rootstock created at Inia from a cross between Solanum lycopersicum and S. chilense. The trial was conducted at the Laboratory of Physiology and Biology and Molecular (Lfbm) of Inia-Rayentué, O'Higgins Region. The hypothesis postulates that the use of growth regulators in vitro micropropagation improves the quality of clonal rootstock seedlings and optimizes the grafting process in tomatoes. A rootstock was micro propagated, using a culture medium with different regulator contents: the control treatment (without growth regulator), indole-3-butyric acid (Iba), 1-naphthaleneacetic acid (Ana), the combination of both in the same concentration (Iba + Ana) and the control treatment (control), which was the Murashige-Skoog (Ms) culture medium without hormones. Contamination, mortality, and vigour (aerial and root growth) were evaluated. The Iba treatment resulted in better quality in vitro seedlings, with better root and aerial growth compared to the other treatments. Therefore, from this work, quality salinity-tolerant clonal seedlings were obtained for grafting tomatoes sensitive to saline stress.

Audience Takeaway:

• It can be used for researchers working in in vitro culture.
• Make better use of growth regulators for in vitro production.
• List all other benefits: Benefits to the plant procurement industry in obtaining quality vitro plant.