HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.
HYBRID EVENT
September 16-18, 2024 | Rome, Italy
GPMB 2024

Kanlaya Kong Ngern

Kanlaya Kong Ngern, Speaker at Plant Biology Conferences
Kanlaya Kong Ngern
Khon Kaen University, Thailand
Title : Identification of salt responsive proteins in rice leaf sheath (Oryza sativa L.) with nanoliquid chromatography-Tandem mass spectrometry

Abstract:

To display the comparative proteomic profile of two rice leaf sheaths under salt stress, seeds of Thai moderately salt tolerant rice (Leaung Anan, LA) and high salt tolerant rice (Pokkali, PX) were grown in hydroponic culture for 21 days before NaCl was introduced initially at the level of 12 dS m-1 for 10 days. Then the leaf sheath proteomes were analyzed by 1D-SDS-PAGE and NanoLC-MS/MS. In this study 873 proteins were detected. Among of these proteins, 219 proteins were known proteins and the other proteins were unnamed and unknown proteins. By using Mev software, we found that only 31 proteins in treated plants of both rice cultivars significantly expressed. Interestingly, we found that both rice cultivars have differing expressed proteins pattern such as putative sodium symporter protein was up-regulated more than 5 times in PX but down-regulated in LA and protein kinase was up-regulated more than 5 times in LA but down- regulated in PX.

Keywords: mass spectrometry, proteomics, rice leaf sheaths, salt stress.

Audience Takeaway:

As a result of my will presentation, the audience will be known the up-regulated proteins more expressed may be play an important role to help them to survival under salt stress.

  • Explain how the audience will be able to use what they learn?                                                     
  • The audience will be able to use 1D-SDS-PAGE and NanoLC-MS/MS techniques to analyzed leaf sheath proteomes.
  • How will this help the audience in their job?                                                                               
  • These techniques will be help the audience to understand the some mechanisms of salt stress through the expression of some inducible proteins.
  • Is this research that other faculty could use to expand their research or teaching?                       
  • Yes, this research could be use to expand to many research or teaching such as in my research group and in my classroom.
  • Does this provide a practical solution to a problem that could simplify or make a designer’s job more efficient?                                                                                                                                           
  • Yes, my results will be shown that some protiens will be up-regulated and may be play an important role to help plants to survival under salt stress.
  • Will it improve the accuracy of a design, or provide new information to assist in a design problem
  • Yes, it will.

Biography:

I am assistant Professor Dr.Kanlaya Kongngern. I have been work in Khon Kaen University since 1997. My teaching and research are in plant stress physiology. My research aims to determine some proteins that were up-regulated and may be play an important role to help plants to survival under salt stress. Some of my research aims to improve rice aroma level by using foliar spraying with stress stimulants and to improve quality of produce of economic crops by using such as H2O2. So far, there are more than 15 graduates finish from me had gone on to work in this field.
 

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