Title : Identification of salt responsive proteins in rice leaf sheath (Oryza sativa L.) with nanoliquid chromatography-Tandem mass spectrometry
Abstract:
To display the comparative proteomic profile of two rice leaf sheaths under salt stress, seeds of Thai moderately salt tolerant rice (Leaung Anan, La) and high salt tolerant rice (Pokkali, Px) were grown in hydroponic culture for 21 days before Nacl was introduced initially at the level of 12 dS m-1 for 10 days. Then the leaf sheath proteomes were analyzed by 1D-Sds-Page and NanoLc-Ms/Ms. In this study 873 proteins were detected. Among of these proteins, 219 proteins were known proteins and the other proteins were unnamed and unknown proteins. By using Mev software, we found that only 31 proteins in treated plants of both rice cultivars significantly expressed. Interestingly, we found that both rice cultivars have differing expressed proteins pattern such as putative sodium symporter protein was up-regulated more than 5 times in Px but down-regulated in La and protein kinase was up-regulated more than 5 times in La but down- regulated in Px.
Keywords: mass spectrometry, proteomics, rice leaf sheaths, salt stress.
Audience Takeaway:
As a result of my will presentation, the audience will be known the up-regulated proteins more expressed may be play an important role to help them to survival under salt stress.
- The audience will be able to use 1D-Sds-Page and NanoLc-Ms/Ms techniques to analyzed leaf sheath proteomes.
- These techniques will be help the audience to understand the some mechanisms of salt stress through the expression of some inducible proteins.
