7th Edition of
Phytochemical Analysis shows the extraction, screening and identification of medicinally active substances in plants. Substances like flavonoids, alkaloids, carotenoids, tannin, antioxidants and phenolic compounds are the phytochemicals derived from plants through the phytochemical process.
Phytochemical Analysis starts with steps of plant collection includes collection of plants, cleaning of plants, drying, powdering and followed by methods of extraction includes plant tissue homogenization, serial exhaustive extraction, soxhlet extraction, maceration, decoction, infusion, digestion, percolation, sonication and ends up with quantitative and qualitative analysis.
Preliminary qualitative analysis tests shows the presence of containing glycosides, phenolic compounds, flavonoids, flavonol glucosides, phytosterols, proteins, saponins, volatile oil, gums and mucilages.
Quantitative and qualitative analysis techniques are Gas Chromatography, High Performance Liquid Chromatography, High Performance Thin Layer Chromatography, and Optimum Performance Laminar Chromatography and for method of detection use UV Spectroscopy, IR Spectroscopy, Mass Spectroscopy, Nuclear Magnetic Resonance Spectroscopy, X-Ray Crystallography.