HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.
September 16-18, 2024 | Rome, Italy
GPMB 2023

Ryan Tay

Ryan Tay, Speaker at Botany Conference
Singapore Institute of Technology, Singapore
Title : Isolation and identification of plant probiotics for leafy greens


Plant probiotic bacteria are plant-associated microorganisms that, when applied in a specific amount, improve the growth and yield of the host plants while also suppressing diseases. These plant probiotics have been shown to improve the production of phytohormones, antibiotics, and lytic enzymes; fix atmospheric nitrogen; and even help in the solubilization of soil mineral nutrients (Rahman et al., 2018). Major general of probiotic bacteria that promote plant growth include Bacillus, Pseudomonas, Acinetobacter, Arthrobacter and Serratia (Rahman et al., 2018). One example of these probiotics being able to increase the yield of the plant is in strawberries, where the bacteria species Bacillus amyloliquefaciens and Paraburkholderia fungorum increased both the size of the fruits as well as the quantity of the leaves and roots (Rahman et al., 2018). Another example would be broccoli, where the inoculation of PGPR bacteria (Bacillus cereus, Brevibacillus reuszeri and Rhizobium rubi) had shown an increment in chlorophyll, N, K, Ca, S, P, Mg, Fe, Mn, Zn and Cu contents. Crop yield increased by 17%, 20.2%, and 24.3% and chlorophyll content by 14.7%, 14.0% and 13.7% in treatments added with Bacillus cereus, Brevibacillus reuszeri and Rhizobium rubi, respectively (Rahman et al., 2018). In addition, some PGPR strains are effective in suppressing pest, pathogen and effective as biocontrol agents. Study reported that various Bacillus strains are important in controlling pathogens and improving plant growth due to antagonistic relationships (Rahman et al., 2018). A previous study also showed that PGPR is involved in the production of plant hormones such as indole acetic acid (IAA). The bacteria species most studied and likely to produce this compound are Bradyrhizobium japonicum SB-1 and Bradyrhizobium thuringiensis (Asghari et al., 2020). This study determined the reproducibility of using three plant probiotics strains (Isolate 4 (Acinetobacter pittii), Isolate 9 (Bacillus altitudinis), and Isolate 8 (Bacillus licheniformis)) on the plant growth of curly kale, Jericho lettuce and Thai basil seeds.


Dr Ryan Tay is an Associate Professor in the Food, Chemical and Biotechnology cluster. He is a Microbiologist by training and has been active in areas related to Food Microbiology and Food Safety. Since joining SIT, he has moved on to research on aquaculture and  urban farming technologies with concentration on the use of microbial probiotics. He works closely with engineering cluster colleagues on research projects funded through the SIT Ignition  grant, MOE-TIF, Temasek Foundation and SFA grants. He is a member of the IRB, IACUC and IAC committees. He also serves as the working committee member in several national level Working Groups in setting up industry standards and technical guidelines, including as the Technical assessor for the Singapore Accreditation Council.