HYBRID EVENT: You can participate in person at Valencia, Spain or Virtually from your home or work.
September 11-13, 2023 | Valencia, Spain
GPMB 2020

Nisreen A AL Quraan

Nisreen A AL Quraan, Speaker at plant science conference
Jordan University of Science and Technology, Jordan
Title : Physiological and Biochemical characterization of GABA shunt pathway in pea Pisum sativum L. seedlings under drought stress


The physiological and biochemical role of γ-aminobutyric acid (GABA) shunt pathway in green pea seedlings (Pisum sativum L.) was studied in response to soil water holding capacity levels: 80%, 60%, 40%, 20% and 10% grown under continuous light at 25ºC for 7 days and 14 days, separately. Characterization of seeds germination pattern, seedlings growth (plant height, fresh weight, dry mass and chlorophyll contents), GABA shunt metabolite levels (GABA, glutamate and alanine), total proteins and total carbohydrates level and oxidative damage (MDA level) were examined. Data showed significant effect of drought stress on seed germination, plant growth, GABA shunt metabolite level, total proteins and carbohydrates contents and MDA level. A significant decline in seed germination percentage was recorded at 20% drought level, which indicated that 20% of soil water holding capacity is the threshold value of water availability for normal germination after 14 days. Seedling fresh weight, dry mass and plant height were significantly reduced with positive correlation as water availability was decreased. There was a significant decrease with positive correlation in Chl a and Chl b content in response to 7 days and 14 days of drought. GABA shunt metabolites were significantly increased with negative correlation as water stress increased. Pea seedlings showed significant increase in protein content as drought stress was increased. Total carbohydrates level increased significantly when amount of water availability decreased. MDA content increased slightly but significant after 7 days and sharply after 14 days under all water stress levels. The maximum increase in MDA content was observed at 20% and 10% water levels. Results presented in this study indicated that pea seedlings suffered minor damage under mild drought stress but severe lipid peroxidation and oxidative damage occurred as water deficit increased. The elevated level of GABA metabolites, soluble sugars and total proteins under water stress might be involve in cellular osmotic adjustment, protecting plants from oxidative stress, balancing of Carbon and Nitrogen (C:N) metabolism and maintaining cell metabolic homeostasis and cell turgor.


Biographical sketch: Nisreen AL-Quraan graduated in 1998 with Bachelor of Science degree from the Department of Biological Sciences, Yarmouk University, Jordan. She joined the graduate program in the Department of Biological sciences, Yarmouk University and received her Master of Science degree in Plant Biochemistry and Molecular biology in 2001. After completion of her MS, she worked as research and teaching assistant for two years in the Department of Biological Sciences, Yarmouk University, Jordan. On May, 2004 she joined the Department of Biological Sciences, Auburn University, Alabama, USA to pursue her PhD degree in Plant Biochemistry and Molecular Biology working on the plant abiotic stress interaction and the role of GABA shunt pathway in plant stress tolerance. She obtained her PhD Degree in August, 2008 from Auburn University, Alabama, USA. Since September 2008, Nisreen AL-Quraan has been working as a professor in plant biochemistry and molecular biology at Jordan University of Science and Technology, JORDAN. Her research is focusing on investigating the pathways that enable plants to adapt and tolerate harsh biotic and Abiotic stress conditions. She is interested in understanding the role of GABA shunt metabolic pathway that is activated in response to the interactions between plants and its environments.

Research interest: Plant Biochemistry and Molecular Biology, Plant and Environment, Stress Physiology