Title : Efficient CRISPR/Cas9 based gene knockout in Chinese cabbage (Brassica rapa subsp. pekinensis)
Abstract:
enome editing offers great advantage to reveal gene function and generate agronomically important mutations to crops. Here, we report the genome of Chinese cabbage (Brassica rapa subsp. pekinensis, AA genome, 2n=20), an important leafy crop, can also be precisely edited by CRISPR/Cas9 system. CRISPR/Cas9 system performed genome editing, such as insertions or deletions at the expected positions of the candidate genes in transgenic Chinese cabbage plants. Furthermore, there were very likely no off-target mutations, indicated by examining regions that were highly homologous to sgRNA sequences. Our results show that CRISPR/Cas9 system is a powerful tool to effectively create knockout mutations in Chinese cabbage.
