Title : The effect IAA and GA3 on coumarin and scopoletin in Artmisia dracunculus L
Abstract:
The genus Artemisia (Asteraceae)is one of the plants that is widely used as a flavoring and traditional medicine in Asian, European and American countries. Numerous compounds, including essential oils and secondary metabolites such as flavonoids, phenylpropanoids, coumarins, are responsible for numerous biological properties, including antioxidants, antitumor, antimicrobial, antifungal, and immunomodulatory properties. These characteristics have led to increased attention to this plant in terms of identifying and extracting effective compounds and biotechnological issues such as micropropagation through issues culture. In recent years, due to its numerous properties and practical aspects, many pharmacological and biochemical studies have been conducted on different species of Artemisia. In this study, from a physiological perspective, an attempt has been made to investigate the effect of various factors, including growth regulators, on the active compounds. Tarragon plants in this experiment were grown under potted conditions in the Varamin region of Iran the samples were arranged in a randomized complete block design with three replications. The aerial parts of the plants were treated with foliar application of IAA (100, 200, 250 µM) and GA3 (50, 100, 150 µM) in various treatments alone or in pairs three times a week. In this work the effect of two growth regulators was investigated by three treatments: A(100IAA+50GA3µM). B(200IAA+100GA3µM) and C(250IAA+150GA3µM). After one week, the aerial parts were harvested and dried, and the phenolic extract was prepared by the Folin-Ciocca-Lescho method for analysis by HPLC the phytochemical compounds of treatments and control were analyzed by HPLC (Knauer UV. detector smartly 2500_s_7700 PUMP).In this study, the amount of coumarin and scopoletin increased in all treatments The use of GA3 alone significantly increased coumarin from 24.62 μg/ml in the control treatment to 31.78 μg/ml in the GA3 treatment (150 μM).The application of IAA alone significantly increased the amount of scopoletin from 10.35 mg/ml in the control sample to 32.27 in the IAA treatment (250 μM) In treatment of C(250IAA+150GA3µM), the amount of coumarin was 27.10 mg/ml and the amount of scopoletin was 32.54 μg/ml, which showed an increase compared to the control. The results of this research show the positive effect of growth regulators on increasing the amount of effective compounds, including phytoalexins, in plants which is probably achieved by stimulating the biosynthetic pathway of phenolic compounds. The use of these growth regulators increases compounds in plants that are important for medicinal, health, cosmetic, and nutritional uses. This method can be used in all plants and helps improve numerous properties, including antioxidant, antimicrobial, and antifungal properties.
