Title : Cichorium intybus local populations biodiversity screening and their importance in ruminant diet
This is a preliminary characterization screening study of a several spontaneous flora populations belonging to the species Cichorium intybus / common chicory and cultivated varieties Spadona and Puna (varieties, already used in the diet of dairy cows due to the high percentage of protein). The samples, individuals belongings to local populations of the species Cichorium intybus / common chicory were collected from the main romanian regions: Maramure?, Moldova, Dobrogea, Muntenia, Banat, Cri?ana, Transylvania and individuals belongings to the Spadona, Puna variants, respectively. It was performed a genetic analysis of nuclear, chloroplastidial, mitochondrial and intergenic genetic markers: ITSs [(ITS-u1 / ITS-u4), ITS1 (transcribed spacer), ITS2 (transcribed spacer), ITS3, ITS4], nad1 / 2–3, cox1, matK, rbcLa, rpoB, rpoC1, psbA-trnH (intergenic spacer-chloroplast), rbcL-accD (intergenic spacer-chloroplast) trnL-trn (intergenic spacer-chloroplast). Biochemical analysis was performed for P, K and N total protein respectively (%). In genetic analysis, the similarity matrices for analyzed molecular markers correlated with the sampling locations and the cultivated varieties Spadona / Puna indicate the Constan?a county population as the closest to the cultivated variety – Spadona and the local populations from Arad/AR Hunedoara/HD, CarasSebe?/CS and Bihor/BH counties exhibits the highest genetic interpopulational similarities. In biochemical analysis, the highest values of nitrogen correlated with protein (%) was presented in Constan?a/CT (2.74 N2 / 17.13% protein) and Sibiu/SB (2.72 N2 / 17.00% protein)populations, even higher than in the cultivated variety (Spadona - 2.13 N2 / 13.31% protein). Concluding, the common chicory from Constan?a and Sibiu counties populations are the closest to the cultivated variety - Spadona, these populations are the best suitable candidates in a possible plant ameliorating program.