Title : Isolation of plant-growth promoting rhizobacteria from mixed-conifer forest in Sierra Nevada, California
Abstract:
Climate change enhances the occurrence of extreme weather: wildfires, drought, rising summer temperatures—all of which dramatically decline forest growth and increase tree mortality in the mixed-conifer forests of Sierra Nevada, California. However, microbiota living in mutualistic relations with plant rhizospheres have been found to mitigate the effects of suboptimal environmental conditions. It is the goal of this research is to isolate native beneficial bacteria—plant-growth promoting rhizobacteria (PGPR)—that can alleviate heat stress in Pinus ponderosa and Pseudotsuga menziesii seedlings. Bacteria was isolated from the rhizosphere of P. ponderosa juveniles located in mixed-conifer stand, and further characterized for PGP potential based on ability to produce key growth regulatory phytohormones including auxin, cytokinin, and gibberellic acid. Out of ten soil samples taken, sixteen colonies were isolated and qualitatively confirmed to produce indole-3-acetic acid (auxin) using Salkowski’s reagent. These bacterial isolates were further analyzed to quantitatively assess auxin, cytokinin, and gibberellic acid production through a variety of spectrophotometric assays. Furthermore, bioassays will be performed to determine isolates abilities to increase tolerance in heat-stressed Pinus ponderosa and Pseudotsuga menziesii seedlings. Upon completion of this research, a PGPR could be utilized to support the growth and transplantation of conifer seedlings as summer temperatures continue to rise due to the effects of climate change.
