De novo organ regeneration, which gives rise to adventitious roots and shoots, is a type of plant regeneration for survival after injury. In Arabidopsis, two main cell fate transition steps are required to establish the root primordium during de novo root root regeneration from leaf explants. The first step from regenerationcompetent cells to root founder cells involves activation of WOX11 and WOX12 expression by auxin. In the second step, WOX11 and 12 directly activate WOX5, WOX7 and LBD16 for initiation of root primordium. We also established a system to study de novo shoot regeneration from Arabidopsis hypocotyls without added hormones. Overall, wound signaling, auxin behavior and cell fate transition are successively involved in de novo organ regeneration.