Title : Transfer of chromatin of wild goatgrasses (Aegilopssp.) carrying leaf rust resistance genes into triticale (Triticosecale Wittmack) using chromosome engineering
I n cereal breeding, precise chromosome engineering using homologous recombination is a method harnessed for controlled introduction of chromatin fragments with desirable genes or loci into nuclear genome of crop plants. Alternatively, this practice can be employed for induction of changes in chromosome structure, which increase the value of crops. This study focuses on introduction of chromosomes (and chromosome segments in further stages) of goatgrasses (Aegilops sp.) carrying leaf rust and yellow rust resistance genes into cultivated triticale using chromosome engineering. In this purpose we have developed three types of monosomic substitution lines of hexaploid triticale (× TriticosecaleWittmack) carrying 2DAe.tauschii or 2SAe. kotschyior 2SAe. variabilis instead of chromosome 2R. At the same time, we have produced triticale DH lines carrying telosomic 2R chromosomes and 5BS-5BL.5RL translocation (lack of Ph1 loci). The aim of this work is to develop three types of translocation plants carrying Lr22a+Lr39 (from Aegilopstauschii), Lr54+Yr37 (from Ae. kotschyi) and Lr59 (from Ae. variabilis). The primary recombinants carrying Robertsonian translocations were produced using induced hybridizations between triticale monosomic plants carrying a donor Aegilopschromosome and triticale (lacking Ph1 loci) bearing 2R telosomic chromosome as an acceptor. The future aims involves the production of secondary recombinants, carrying reduced segment of Aegliops chromatin and the consolidation of the resistance genes loci.