Online Event
September 01-03, 2022 | Online Event
GPMB 2018

Tapetum PCD retardation is critical for amino acid biosynthesis and aliphatic metabolism during sesame anther development

Ting Zhou, Speaker at Plant Science Conference
Oil Crops Research Institute, Chinese Acdamy of Agricultural Sciences, China
Title : Tapetum PCD retardation is critical for amino acid biosynthesis and aliphatic metabolism during sesame anther development

Abstract:

Anther development and male fertility was critical for crop seed formation and yield production. Male sterile plants provide an efficient pollination control system for hybrid breeding. Sesame 95ms-5AB was a novel recessive genic male sterile two-type line controlled by a single RGMS gene. The male abortion of 95ms-5A was appeared after tetrad stage. iTRAQ analysis and Metabolomics analysis between sterile anthers (95ms-5A) and fertile anthers (95ms-B) were performed to unravel the mechanisms of 95ms-5 male sterility. iTRAQ analysis indicated that differential expressed proteins (DEPs) were mainly involved in programed cell death (PCD), amino acid biosynthesis and lipid metabolism. Tunnel analysis further confirmed that tapetal cell degeneration was delayed in male sterile plant. Callose staining analysis indicated that callose degradation was abnormal in sterile plants during anther development. Lipid staining analysis revealed that lipid accumulation was higher in sterile anthers as compared with fertile anthers. TEM observation further confirmed the delayed tapetum PCD and the pollen wall polymerization and patterning were disturbed in sterile anthers. Metabolomic analysis as well revealed that differential metabolites between sterile anthers and fertile anthers were mainly accumulated in amino acid biosynthesis and fatty acid metabolism. Comparing to fertile anthers, the content of amino acid was reduced in tetrad stage, while was increased in microspore stage in sterile anthers and the branched amino acids were mainly altered in sterile anthers. Metabolomic analysis also confirmed the lipid content, mainly the fatty acid content, was altered in sterile anthers as compared with fertile anthers. This study demonstrated that delayed tapetum PCD disturbed amino acid biosynthesis and fatty acid metabolism, leading to male gametogenesis inhibited in sesame. Our study provides a first insight into the mechanism that result in genetic male sterility in sesame and contributes to a better understanding of the regulatory network involved in anther development in sesame.

Biography:

Ting Zhou, female, Ph. D, graduated from Huazhong Agricultural University, working in Oil Crops Research Institute, Chinese Academy of Agricultural Sciences. The research field was mainly focus on gene cloning and functional verification of important agronomic traits of sesame. Published papers in molecular & cellular proteomics, plant molecular biology, BMC plant biology. Hosting a national natural science foundation youth fund.

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