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September 01-03, 2022 | Paris, France
GPMB 2018

Stimuli from prey induce expression of digestive enzymes in the carnivorous plant of the genus Nepenthes

Andrej Pavlovic, Speaker at Plant Science Conferences
Palacky University, Czech Republic
Title : Stimuli from prey induce expression of digestive enzymes in the carnivorous plant of the genus Nepenthes

Abstract:

Carnivorous plants of the genus Nepenthes have evolved passive pitcher traps for prey capture. In this study we investigated the ability of chemical signals from prey (chitin, protein, ammonium) to induce transcription and synthesis of digestive enzymes in carnivorous plant Nepenthes x Mixta. We used real-time PCR and specific antibodies generated against aspartic proteases nepenthesins, type III and type IV chitinases to investigate the induction of digestive enzyme synthesis in response to different chemical stimuli from prey. Transcription of nepenthesins was strongly induced by ammonium, protein and live prey; chitin induced transcription only very slightly. This is in accordance with amount of released enzyme and proteolytic activity in digestive fluid. Although, transcription of type III chitinase was induced by all investigated stimuli, significant accumulation of enzyme in digestive fluid was found mainly after protein and live prey addition. Protein and live prey were also the best inducers for accumulation of type IV chitinase in digestive fluid. Although ammonium strongly induced transcription of all investigated genes probably through membrane depolarization, strong acidification of digestive fluid affected stability and abundance of both chitinases in digestive fluid. The study showed that the proteins are universal inductors of enzyme activities in carnivorous pitcher plant best mimicking the presence of insect prey. This is not surprising, because proteins are much valuable source of nitrogen than chitin. Extensive vesicular activity was observed in prey-activated glands using transmission electron microscopy.

Biography:

Andrej Pavlovič is an associated professor in the Department of Biophysics at the Palacký University in Olomouc, Czech Republic. He received his Ph.D. in plant physiology from the Faculty of Natural Sciences of Comenius University in Bratislava, Slovakia. In 2015, he defended his habilitation thesis entitled: Cost/benefit model for the evolution of botanical carnivory: from molecules to ecology. His research has focused on photosynthesis, digestive enzymes and nutrient uptake in carnivorus plants. Currently, his main area of interest is regulation of digestive enzyme secretion in the carnivorous plants. He is the author of ≈ 30 peer-reviewed papers, associate editor of journal Photosynthetica, and a national representative to The Federation of European Societies for Plant Biology (FESPB).

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