Online Event
September 01-03, 2022 | Online Event
GPMB 2018

Regulatory divergence in wound-responsive gene expression in domesticated and wild tomato

Ming Jung Liu, Speaker at Plant Science Conference
Academia Sinica, Taiwan
Title : Regulatory divergence in wound-responsive gene expression in domesticated and wild tomato


The evolution of transcriptional regulatory mechanism is central to how stress response and tolerance differ between species. However, it remains largely unknown how divergence in cis-regulatory sites and, subsequently, transcription factor (TF) binding specificity contribute to stress-responsive expression divergence, particularly between wild and domesticated species. By profiling wound-responsive gene transcriptomes in wild Solanumpennellii and domesticated S. lycopersicum, we found extensive wound-response divergence and identified 493 S. lycopersicum and 278 S. pennellii putative cis-regulatory elements (pCREs) that were predictive of wound-responsive gene expression. Only 24-52% of these wound response pCREs (depending on wound-response patterns) were consistently enriched in the putative promoter regions of would-responsive genes. In addition, between these two species, their differences in pCRE site sequences were significantly and positively correlated with differences in wound-responsive gene expression. Furthermore, ~11-39% of pCREs are specific to only one of the species and likely bound by TFs from different families. These findings indicate substantially regulatory divergence in these two plant species diverged ~3-7 million years ago. Our study provides new insights into the mechanistic basis of how the transcriptional response to wounding is regulated and, importantly, the contribution of cis-regulatory components to variation in wound-responsive gene expression between a wild and a domesticated plant species.


I am a plant molecular and computational biologist with strong interest in stress biology. After completing the postdoctoral research at MSU, USA, I started my own lab in May 2016 and currently am an Assistant Research Fellow in Academia Sinica, Taiwan. Our research directions are currently focusing to (1) develop the combining experimental and mathematical modeling methods for genome-wide annotation and quantification of translated genes and (2) systematically identify the transcriptional/translational control in response to stress in crops.