Zhuang Yong

Title : Comparative transcriptome analysis reveals key genes and pathways related to male sterile of eggplant (Solanum melongena L.)

Abstract:

Male sterile (MS) is an effective tool for hybrid production. We have obtained eggplant male sterile lines from progenies of an interspecific hybrid (S. aethiopicum × S. melongena). To understand the mechanism for MS in eggplant, the comparative transcriptomic files of MS line and its maintainer line was analyzed with the RNA-seq technology. A total of 117695 unigenes were assembled and 19652 differentially expressed genes (DEGs) were obtained based on FPKM method. The results showed 1716 DEGs were shared in three groups. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these DEGs were mainly involved in oxidation-reduction, carbohydrate and amino acid metabolism. Moreover, transcriptional regulatory was also the impact effector for MS and anther development. Weighted gene co-expressed network analysis (WGCNA) showed two modules might be responsible for male sterile. This study threw light on the molecular mechanism of MS and identifying several key genes related to male sterile.

Biography:

Dr. Zhuang has been with Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, China since 1996. He serves as the principle investigator for eggplant genetics and breeding. He conducts research to characterize the inheritance of economically important attributes in eggplant and develop improved breeding lines and cultivars. He earned his Ph.D. in vegetable genetics and breeding at Nanjing Agricultural University, China. His research work was supported by the National Key R&D Program and Natural Science Foundation of China , and Key R&D Program and Natural Science Foundation of Jiangsu Province.