Title : Comparative transcriptome analysis reveals key genes and pathways related to male sterile of eggplant (Solanum melongena L.)
Male sterile (MS) is an effective tool for hybrid production. We have obtained eggplant male sterile lines from progenies of an interspecific hybrid (S. aethiopicum × S. melongena). To understand the mechanism for MS in eggplant, the comparative transcriptomic files of MS line and its maintainer line was analyzed with the RNA-seq technology. A total of 117695 unigenes were assembled and 19652 differentially expressed genes (DEGs) were obtained based on FPKM method. The results showed 1716 DEGs were shared in three groups. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these DEGs were mainly involved in oxidation-reduction, carbohydrate and amino acid metabolism. Moreover, transcriptional regulatory was also the impact effector for MS and anther development. Weighted gene co-expressed network analysis (WGCNA) showed two modules might be responsible for male sterile. This study threw light on the molecular mechanism of MS and identifying several key genes related to male sterile.