HYBRID EVENT: You can participate in person at Valencia, Spain or Virtually from your home or work.
September 11-13, 2023 | Valencia, Spain
GPMB 2017

Aline Koch

Aline Koch, Speaker at Plant Science Conference
Justus Liebig University, Germany
Title : RNA spray fights fungus


Meeting the increasing food and energy demands of a growing population will require (the development of) developing ground-breaking strategies that promote sustainable plant production. RNA interference has emerged as a powerful genetic tool for scientific research. The demonstration that agricultural pests, such as insects and nematodes, are killed by exogenously supplied RNA targeting their essential genes has raised the possibility that plant predation can be controlled by lethal RNA signals. Host-induced gene silencing has shown great potential for controlling pest and diseases in crop plants (Koch et al. 2013, Koch and Kogel 2014, Abdellatef et al. 2015). However, while delivery of inhibitory noncoding double-stranded (ds)RNA by transgenic expression is a promising concept, it requires the generation of transgenic crop plants which may cause substantial delay for application strategies depending on the transformability and genetic stability of the crop plant species. Using the agronomically important barley - Fusarium graminearum pathosystem, we alternatively demonstrate that a spray application of a long noncoding dsRNA (791 nt CYP3-dsRNA), which targets the three fungal cytochrome P450 lanosterol C-14α-demethylases, required for biosynthesis of fungal ergosterol, inhibits fungal growth in the directly sprayed (local) as well as the non-sprayed (distal) parts of detached leaves (Koch et al. 2016). Unexpectedly, efficient spray-induced control of fungal infections in the distal tissue involved passage of CYP3-dsRNA via the plant vascular system and processing into small interfering (si)RNAs by fungal DICER-LIKE 1 (FgDCL-1) after uptake by the pathogen. We discuss important consequences of this new finding on future RNA-based disease control strategies. Given the ease of design, high specificity, and applicability to diverse pathogens, the use of target-specific dsRNA as an anti-fungal agent offers unprecedented potential as a new plant protection strategy, thereby opening novel avenues for improving crop yields in an environmentally friendly and sustainable manner.


Dr. Aline Koch studied Biology at the Justus-Liebig University, Giessen and graduated as MS in 2010. Since then she joined the research group of Prof. Karl-Heinz Kogel at the Institute of Phytopathology. She received her PhD degree in 2013 at the same institution. At this time, she was one of the first scientists working in the field of RNAi-based plant protection. In the first year of her postdoc, she additionally developed a non-GMO RNA spray approach (SIGS) in cooperation with BASF. Since 2014 she is a group leader with the research focus: RNAi-silencing mediated plant immunity.