Title : Expression of Heavy Metal ATPase HMA1 and HMA2 in Brassica juncea and Brassica nigra grown at Different Levels of Cu and Cd.
Abstract:
Brassica species are well known as metal accumulators and some of them are being used for phytoremediation in contaminated soils. Approximately 25% of the documented metal hyper accumulating species have been found in Brassicacae family. In this report we have evaluated the accumulation capacity of two well known Brassica sps (B. juncea and B. nigra) grown at different levels of Cu and Cd. Heavy metal transporting proteins have an important role in maintenance of metal homeostasis in plants and are involved in a number of different processes including metal uptake, delivery of metals to cellular compartments and metal detoxification. P1B-ATPases are one of the largest and diverse protein families in membrane proteins responsible for transporting metals from roots to shoots. We have done the phylogenetic analysis of these proteins by using the sequence data of these genes from B. juncea. Homology analysis of these genes were proformed by using different data banks. Additionally we have analyzed the gene expression pattern of metal ATPases (HMA1 and HMA2) in plant leaves and roots grown at different levels of Cu and Cd by using R-T PCR. Our data showed that HMA1 and HMA2 play an important role in metal accumualtion and detoxification in B. juncea leaves. Over all our aim in this project is to find out the Cu and Cd induced gene regulation in accumulator plant B. nigra and tolerant plant B. juncea.
