Title : De novo Assembly and TranscriptomeCharacterization of Pineapple (Ananascomosus) Flower Induction in Response to Ethephon
Abstract:
Pineapple (Ananascomosus) is one of the most important fruit trees in tropical and subtropical areas. The flowering rate of pineapple is easy to be influenced by external environmental factors, and the induction of floral bud by application of ethephon (ETH) is widely used in commercial production to enhance flowering ratio and regulate the time to market of pineapple. In the induction of floral buds, ETH plays complex and variable roles in pineapple. However, lack of information hinders our understanding of the mechanisms of flower bud differentiation induced by ETH in pineapple. Here, high-throughput RNA sequencing (RNA-Seq) was employed for de novo assembly and transcriptome characterization of pineapple (Ananascomosus) flowering in response to ethephon. More than 80 million base pair-end reads were generated and assembled into 41287 unigenes with an average length of 802bp. These unigenes were annotated by query against various public databases. Approximately, 21,575 (52.26%) unigenes annotated by the Nr database and15220 (36.86%) unigenes annotated based on known proteins in the Swiss-Prot database, 11543 (25.54%) unigenes were annotated based on Gene Ontology terms, and 7868 (19.06%) unigenes were assigned to Kyoto Encyclopedia of Genes and Genomes pathways, 13542 and 11213 unigenes were annotated by Pfam and String database respectively. In addition, Many known and novel ETH-induced and flowering-induced genes were found, in which 12 unigenes were randomly selected for more detailed expression comparison responding to ETH. These studies provide the global expression analysis of flowering-induced transcripts and a robust database for investigating the functions of genes induced by ETH in pineapple
Keywords: Pineapple, Ethephon, Flowering-Induction, RNA-Seq
