Title : Detection of specific mutation conferring resistance in fungal pathogens in barely
The key hypothesis of the study was real-time PCR (qPCR) assessment of plant samples to measure ratio of mutant and wild alleles. The aim of this study was to detect the resistance of chosen fungal isolates collected in the Czech Republic to fungicides by laboratory agar dilution biotest and molecular methods as qPCR. Due to a frequent application of fungicides on the territory of the Czech Republic, the amount of fungicide resistant populations highly increased. Newly developed method in this study can improve and fasten diagnostic and monitoring of the resistance dynamic.