Online Event
September 01-03, 2022 | Online Event
GPMB 2022

Sustainable Low-Cost Surfactin Production And Optimization By Bacillus Subtilis SNW3, Product Characterization, And Its Suitability For Plant Growth Promotion And Bioremediation of Crude Oil

Aneeqa Zafar, Speaker at plant science conferences
Quaid I Azam University, Islamabad, Pakistan
Title : Sustainable Low-Cost Surfactin Production And Optimization By Bacillus Subtilis SNW3, Product Characterization, And Its Suitability For Plant Growth Promotion And Bioremediation of Crude Oil

Abstract:

At present time, every nation is absolutely concern about increase agricultural production and bioremediation of petroleum contaminated soil. Hence, with this intention in current study potent natural surfactant (surfactin) was evaluated for low-cost production by Bacillus subtilis SNW3, previously isolated from Fimkessar oil field, Chakwal Pakistan. The best results were obtained using substrates in combination (white beans powder (6% w/v) plus waste frying oil (1.5% w/v) and (0.1% w/v) urea) with surfactin production of about 1.17 g/L contributing 99% reduction in cost required for medium preparation. To the best of our knowledge, no single report is present describing surfactin production by Bacillus subtilis using white beans powder as a culture medium. Surfactin was confirmed as the principal product characterized by thin-layer chromatography (TLC) and Fourier-transform infrared spectroscopy (FTIR). Additionally, produced surfactin display great physicochemical properties of surface tension reduction value (SFT=28.8 mN/m), significant oil displacement activity (ODA=4.9 cm), excessive emulsification ability (E24=69.8 %), and attains critical micelle concentration (CMC) value at 0.58 mg/mL. Furthermore, surfactin exhibits excellent stability over an extensive range of pH (1-11), salinity (1- 8%), temperature (20-121°C) and even after autoclaving. Subsequently, surfactin produced proved suitable for bioremediation of crude oil (86%) and as potent plant growth-promoting agent that significantly (P<0.05) increase seed germination increase seed germination and plant growth promotion of chili pepper, lettuce, tomato and pea maximum at concentration of (0.7 g/100 mL), proved as potential agent for agriculture and bioremediation processes by lowering economic and environmental stress

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