Online Event
September 01-03, 2022 | Online Event
GPMB 2022

Compatibility and synergistic interactions of fungi, Metarhizium anisopliae, and insecticide combinations against cabbage aphid and cotton aphid species

Ahmad Nawaz, Speaker at Plant science conference
University of Agriculture, Pakistan, Pakistan
Title : Compatibility and synergistic interactions of fungi, Metarhizium anisopliae, and insecticide combinations against cabbage aphid and cotton aphid species


Aphids are major pests affecting cereals, vegetables, fruit, forestry and horticultural produce. A multimodal approach may be an effective route to controlling this prolific pest. We assessed the individual and combined effect of eight insecticides and the entomopathogenic fungi, Metarhizium anisopliae (Metschin.) against cabbage aphid and cotton aphid species under laboratory conditions. Six of the insecticides tested were found to be highly compatible (flonicamid, imidacloprid, nitenpyram, dinotefuran, pyriproxyfen and spirotetramat), showing positive integration with the fungus and were selected for bioassays. The combination mixtures (1:1 ratio of M. anisopliae: insecticide) were significantly more toxic to aphid species than individual treatments. Maximum mortality of cotton aphid (91.68%) and cabbage aphid (88.36%) recorded with combination of flonicamid and M. anisopliae (2.4 × 106 cfu/ml) 72 h after application. While minimum mortality 17.08% and 16.66% was observed with the individual treatment of M. anisopliae (2.4 × 106 cfu/ml) against cotton aphid and cabbage aphid respectively. Our study indicates that M. anisopliae has the potential to control cotton aphid and cabbage aphid within short period of time when combined with insecticides. The combined insecticide M. anisopliae were consistently more toxic than individual treatments. The present study utilized co-toxicity coefficients and synergy factors to calculate the efficacies of different insecticides + M. anisopliae formulations. The toxicity of insecticides, based on their L C50 and L C90 values increased when mixed with M. anisopliae. The insecticides revealed toxicity consistent with their compatibility with M. anisopliae, ranking for efficacy exactly as they did for compatibility. In addition, the synergy factor (SF) and co-toxicity coefficient (CTC) values indicated synergistic interactions at different time intervals. The high values of co-toxicity coefficients, which were accompanied by insect mortalities > 90% for some treatments, illustrate the effectiveness of this dual-attack method of insect pest control. The synergistic efficacy revealed the potential of fungus-insecticide integration against sucking insect pests.


Dr. Ahmad Nawaz studied agriculture with specialization in Entomology at University of Agriculture Faisalabad (UAF), Pakistan and did master degree in biological control in 2008 from Agrocampus Ouest Renne, France. He received PhD degree in 2012 by working in laboratory of Toxicologie Cellulaire et Moléculaire des Xénobiotiques. Centre de Recherche INRA AGROBIOTECH 400 Route des Chappes, 06903, Sophia Antipolis, France. During his PhD he got the opportunity to work with Dr. Roger Rahmani and Dr. George De Sousa. He joined UAF as Assistant Professor in October 2012 and now promoted as associate Professor. He has published more than 30 publication.