Elkharrassi Youssef

Elkharrassi Youssef

Title: Title: Application of molecular biology in the determination of genetic diversity within the genus cactus (Opuntia spp) and identification of the main biomolecules derived from several species of nutritional, therapeutic and medicinal interest.

Elkharrassi Youssef

University of Burgundy, Morocco

Biography

Youssef El Kharrassi is a Doctor of life sciences specialty biochemistry, molecular and cellular biology, and he is doing a thesis in cotuelle between the University of Burgundy Franche-Comté, FS of Gabriel Dijon, Laboratory of BioPeroXIL, Dijon France and University Hassan 1st FST in Laboratory Biochemistry and Neuroscience of Settat Morocco. He is also a Researcher at the National Institute of Agronomic Research Settat Morocco. He has published more than 10 papers in reputed journals Indexed and impacted, and he has more than 40 papers in national and international congresses. Currently, his research is focused on Chemistry of natural substances: the valorization of the isolated secondary metabolites of plants and Study of the effects of natural molecules, to prevent the metabolic, oxidative and/or inflammatory diseases.

Abstract

In Morocco, the cactus is experiencing a growing interest and an unprecedented extension, especially within the framework of the Plan Morocco Green. However, the national germplasm in this crop remains little characterized from the agronomic, systematic and physico-chemical point of view in order to better target its exploitation and its use. Despite the great importance of the cactus in Morocco, its genetic diversity is hardly studied, and the search for new sources of natural molecules with antioxidant potential is of growing interest to industrialists. The objective of our work was to evaluate the genetic diversity of Opuntia species From the experimental site of INRA-CRRA Settat Ain Nzagh, which contains 126 ecotypes harvested from several regions of the kingdom and installed under the same edapoclimatic conditions.with the morphological descriptors and the molecular markers ISSR and RAPD, as well as the characterization of the main biomolecules of medicinal interest, namely phenolic compounds (polyphenol / flavonoids) by Method Folin-Ciocalteau / Depheur and phytosterols/Fatty Acid by Gaz Chromatograhy. All this work will lead us to elucidate the antioxidant and anti-inflammatory effect of the different parts of the cactus plant according to the species, by the method of the DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power). The results show that the identification of seven species, and the cladode of the different species identified has a great potential antioxidant compared to the flower and the spines.