Javier Terol

Javier Terol

Title: Sequencing of 260 Citrus Varieties: Linking Genotypes to Phenotypes

Javier Terol

Centro de Genomica, IVIA, Spain

Biography

Javier Terol been involved in plant genomics since 1997, when he participated in the sequencing of the genome o the model plant Arabidopsis thaliana, the first plant genome obtained. Since 2003 he have been working at the Genomics Centre at the IVIA, developing genomic tools for the generation of new citrus varieties. His team at the IVIA had a leading role in the sequencing of the genome of C. clementina, which has become the reference genome for the Citrus genus. He is involved now in GBS and GWAs studies, in order to ¡obtain markers suitable for their use in the generation of new citrus varieties.

Abstract

Elucidation of genomic sequences is an essential and necessary first step to identify genetic variants associated with agronomic traits of interest. The Citruseq Consortium, a joint effort developed by public institutions and private companies has sequenced the genomes of 260 citrus varieties with the Illumina platform at high average coverage. Paired end genomic DNA libraries of 100 (2x) bp reads and with an average insert size of 500 bp were constructed. The haploid genome of Citrus clementine (www.phytozome.com) was used as reference genome. More than 260 Citrus genomes including rootstocks and species of mandarins, oranges, lemons, grapefruits and limes were sequenced. The consortium also generated a web portal that allows searching and sequence comparisons among approximately 8 million of gene variants and 45 billion of base pairs. The stored information can help unravel fundamental aspects of biological and evolutionary interest such as the origin of citrus. From the commercial standpoint this information may help to authenticate varieties, to improve marker-assisted breeding and to identify genes of major agronomic interest.